NOVITÀ

News del 06-07-2017

Bcl-2: apoptosis checkpoint family

Queste proteine governano la permeabilità della membrana mitocondriale esterna, e possono essere sia pro-apoptotiche (Bax, Bak, Bad, Bim e altre), sia anti-apoptotiche (tra queste Bcl-2, Bcl-xL e Mcl-1) (Figura 1). L'attività dei membri della famiglia Bcl-2 può dunque avere effetti enormi sulla sopravvivenza cellulare, e ciò li rende bersagli terapeutici ottimali.

Nel listino Prodotti Gianni trovi oltre 700 prodotti dedicati a questa famiglia. Contattaci per ricevere la raccolta completa.

Di seguito troverete marker, attivatori e inibitori che Abcam propone in relazione a questa famiglia (compreso Venetoclax, recentemente approvato dalla FDA e dalla Commissione Europea).

Marker per la detection delle proteine della famiglia Bcl-2

Bcl-2 family target	Marker	Species reactivity	Key features
Bcl-2	Anti-Bcl-2 antibody [E17] (ab32124)	Human	Validated for WB, IP, IHC, and IF/ICC. Available in PBS only and conjugated form.
Bcl-2	Anti-Bcl-2 antibody [EPR17509] (ab182858)	Mouse, human	Validated for WB, ICC/IF, flow cytometry, IHC-P
Bcl-XL	Anti-Bcl-XL antibody [E18] (ab32370)	Mouse, rat, human	Recognizes Bcl-xl, Bcl-xS and Bcl x (beta) but does not cross-react with other Bcl-2 members. Validated for WB, IP, IHC and IF/ICC
Mcl-1	Anti-MCL1 antibody [Y37] (ab32087)	Mouse, rat, human	Validated for ICC/IF, WB, IHC-P, flow cytometry, IP. Does not cross-react with other Bcl-2 members
Bax	Anti-Bax antibody [E63] (ab32503)	Mouse, rat, human, Chinese hamster	Validated for IP, WB, IHC-P, sELISA. Knockout validated. PBS only version available
Bak	Anti-Bak antibody [Y164] (ab32371)	Human	Validated for WB, IHC-P, flow cytometry, ICC/IF, IP. HRP conjugate
Bak	Anti-Bak antibody [AT8B4] (ab104124)	Mouse, human	Validated for WB, ELISA, ICC/IF
Bad	Anti-Bad antibody [Y208] (ab32445)	Mouse, rat, dog, human	Validated for flow cytometry, ICC/IF, IHC-P, IP, WB
Bad	Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)	Mouse, rat, human	Detects phosphorylated Bad at S112. Validated for dot blot, WB, IP.
Bim	Anti-Bim antibody [Y36] (ab32158)	Mouse, rat, human	Validated in flow cytometry, ICC/IF, IHC-FoFr, IHC-P, IP, WB

Inibizione e attivazione delle proteine della famiglia Bcl-2

Bcl-2 family target	Modifier	Activity	Key features
Bcl-2	Venetoclax ABT -199 (ab217298)	Inhibitor	· Selective Bcl-2 inhibitor · BH3-mimetic · FDA-approved
Bcl-xl	Z36 (ab141757)	Inhibitor	· Novel Bcl-xl inhibitor · Inhibits cell proliferation and induces autophagy but not apoptosis
Mcl-1	AT 101 (ab145624)	Inhibitor	· BH3-mimetic · Pro-apoptotic agent
Bcl-2, Bcl-xl and Bcl- w	ABT-737 (ab141336)	Inhibitor	· BH3 mimetic: binds Bcl-2 and Bcl-xl with high affinity · Shows antitumor effects in vivo
Pan	Gambogic acid (ab145183)	Inhibitor	· Apoptotic agent · Decreases Bcl-2 and increases Bax expression by competitive inhibition
Bax	BAM7 (ab218116)	Activator	· Selective Bax activator · Targets proapoptotic bax at its trigger site · Induces apoptosis in vitro